Protein Variants | Comment | Organism |
---|---|---|
D399A | 90% of the dehydrogenase activity of the wild-type enzyme | Rattus norvegicus |
E398A | 90% of the dehydrogenase activity of the wild-type enzyme | Rattus norvegicus |
F384_V405del | no dehydrogenase activity | Rattus norvegicus |
G397A | as active as wild-type enzyme | Rattus norvegicus |
K394A | 75% of the dehydrogenase activity of the wild-type enzyme | Rattus norvegicus |
K394A/L395A/R396A/G397A/E398A/D399A | no dehydrogenase activity | Rattus norvegicus |
K394_D399del | no dehydrogenase activity | Rattus norvegicus |
L395A | 80% of the dehydrogenase activity of the wild-type enzyme | Rattus norvegicus |
R396A | as active as wild-type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P28037 | - |
- |
Purification (Comment) | Organism |
---|---|
intermediate domain and its mutants | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Rattus norvegicus |
4°C, stable for 2 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | the enzyme is composed of three domains and possesses three catalytic activities but has only two catalytic centers. The amino-terminal domain (residues 1-310) bears 10-formyltetrahydrofolate hydrolase activity, the carboxyl-terminal domain (residues 420-902) bears an aldehyde dehydrogenase activity, and the full-length FDH produces 10-formyltetrahydrofolate dehydrogenase activity | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
10-formyltetrahydrofolate dehydrogenase | - |
Rattus norvegicus |
FDH | - |
Rattus norvegicus |