Cloned (Comment) | Organism |
---|---|
expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C707A | mutant with a complete loss of dehydrogenase activity of 10-formyltetrahydrofolate and propanal, but hydrolase activity and binding of NADP+ is unchanged | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99000 | - |
4 * 99000, wild-type FDH and C707A mutant | Rattus norvegicus |
393200 | - |
C707A mutant FDH, molecular size detector employing laser-light scattering methodology | Rattus norvegicus |
398800 | - |
wild-type FDH, molecular size detector employing laser-light scattering methodology | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of a C707A mutant of FDH | Rattus norvegicus |
purification of recombinant liver FDH | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | Cys-707 is a key residue of the dehydrogenase active site and acts as nucleophile in the formation of an enzyme-linked thiohemiacetal intermediate | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Rattus norvegicus | ? | - |
? | |
additional information | 10-formyltetrahydrofolate dehydrogenase/hydrolase activities occur at the same time and are associated with separate active sites | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 99000, wild-type FDH and C707A mutant | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus |