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Literature summary for 1.5.1.6 extracted from

  • Krupenko, S.A.; Oleinik, N.V.
    10-Formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells (2002), Cell Growth Differ., 13, 227-236.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the cDNA encoding FDH Rattus norvegicus
cloning of the cDNA encoding FDH, cells from several tumor cell lines are transfected with FDH cDNA cloned into pcDNA 3.1+ vector using LipofectAMINE, expression of FDH inhibits the proliferation of the cell lines as a result of its enzymatic activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
C707A
-
Rattus norvegicus
C707A catalytically inactive mutant Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus
-
tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O Homo sapiens regulation of the ratio of 10-formyltetrahydrofolate to tetrahydrofolate and of the purine biosynthesis through control of the level of 10-formyltetrahydrofolate, down-regulation of FDH in tumors is one of the cellular mechanisms that enhance proliferation, FDH possesses tumor specific suppressor effects tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
male rats
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell significantly decreased levels of FDH and its mRNA in 15 tested tumors compared with normal tissues, strong down-regulation of FDH on both the mRNA and protein level in tumors Homo sapiens
-
brain moderate FDH mRNA level Homo sapiens
-
DU-145 cell
-
Homo sapiens
-
heart
-
Rattus norvegicus
-
heart moderate FDH mRNA level Homo sapiens
-
HEK-293 cell transformed embryonic kidney cell line HEK-293A Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
kidney
-
Rattus norvegicus
-
kidney high FDH mRNA level, specialized organ of folate metabolism Homo sapiens
-
liver specialized organ of folate metabolism Homo sapiens
-
liver high FDH mRNA level Homo sapiens
-
liver high FDH mRNA level Rattus norvegicus
-
lung moderate FDH mRNA level Homo sapiens
-
additional information FDH expression is highly tissue specific, very low or nondetectable levels of FDH mRNA in placenta, spleen, colon, small intestine, and leukocytes Homo sapiens
-
muscle moderate FDH mRNA level in skeletal muscle Homo sapiens
-
ovary moderate FDH mRNA level Homo sapiens
-
pancreas high FDH mRNA level Homo sapiens
-
PC-3 cell
-
Homo sapiens
-
PPC-1 cell
-
Homo sapiens
-
prostate gland moderate FDH mRNA level Homo sapiens
-
testis moderate FDH mRNA level Homo sapiens
-
thymus moderate FDH mRNA level Homo sapiens
-
Tsu-Pr1 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
-
Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O
-
Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O regulation of the ratio of 10-formyltetrahydrofolate to tetrahydrofolate and of the purine biosynthesis through control of the level of 10-formyltetrahydrofolate, down-regulation of FDH in tumors is one of the cellular mechanisms that enhance proliferation, FDH possesses tumor specific suppressor effects Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
?

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Homo sapiens
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
NADP+
-
Homo sapiens
NADP+
-
Rattus norvegicus
tetrahydrofolate
-
Homo sapiens
tetrahydrofolate
-
Rattus norvegicus