Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 0.1 M, strong inhibition | Sus scrofa | |
iodoacetamide | inhibition reversed by 2-mercaptoethanol | Sus scrofa | |
additional information | - |
Rattus norvegicus | |
additional information | not inhibited by (+)-10-formyltetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, folate, aminopterin, tetrahydroaminopterin | Sus scrofa | |
p-chloromercuribenzoate | inhibition reversed by 2-mercaptoethanol | Sus scrofa | |
tetrahydrofolate | - |
Rattus norvegicus | |
tetrahydrofolate | strong product inhibition by both isomers | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
NADP+ | - |
Sus scrofa | |
0.0082 | - |
10-formyltetrahydrofolate | (-)-isomer | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
320000 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | less activity | Rattus norvegicus | - |
kidney | less activity | Rattus norvegicus | - |
liver | - |
Gallus gallus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
spleen | less activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.98 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-15°C, presence of 0.01 mM 2-mercaptoethanol, several weeks, stable | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
ir | |
10-formyltetrahydrofolate + NADP+ + H2O | calculated equilibrium constant: 160000000, specific for (-)-10-formyltetrahydrofolate and NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
ir | |
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | not as substrate: (+)-10-formyltetrahydrofolate | Sus scrofa | ? | - |
? | |
additional information | not as substrate: 5-formyltetrahydrofolate | Sus scrofa | ? | - |
? | |
additional information | enzyme catalyzes hydrolytic cleavage of 10-formyltetrahydrofolate to formate and tetrahydrofolate in absence of NADP+ (15-30% of the rate of oxidative reaction) | Sus scrofa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rattus norvegicus |
7.8 | - |
broad pH-optimum centers at pH 7.8 | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | more than 60% of maximal activity at pH 6.5 and 8.5 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
NAD+ | less than 5% of the activity with NADP+ | Sus scrofa | |
NADP+ | specific for | Rattus norvegicus | |
NADP+ | specific for | Sus scrofa | |
tetrahydrofolate | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
tetrahydrofolate | (-)-isomer | Sus scrofa | |
0.01 | - |
tetrahydrofolate | (+)-isomer | Sus scrofa |