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Literature summary for 1.5.1.6 extracted from

  • Kutzbach, C.; Stokstad, E.L.R.
    10-Formyl tetrahydrofolate:NADP oxidoreductase (1971), Methods Enzymol., 18B, 793-798.
No PubMed abstract available
Search for more literature for 1.5.1.6

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine 0.1 M, strong inhibition Sus scrofa
iodoacetamide inhibition reversed by 2-mercaptoethanol Sus scrofa
additional information
-
 Rattus norvegicus
additional information not inhibited by (+)-10-formyltetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, folate, aminopterin, tetrahydroaminopterin Sus scrofa
p-chloromercuribenzoate inhibition reversed by 2-mercaptoethanol Sus scrofa
tetrahydrofolate
-
 Rattus norvegicus
tetrahydrofolate strong product inhibition by both isomers Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 NADP+
-
 Sus scrofa
0.0082
-
 10-formyltetrahydrofolate (-)-isomer Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart less activity Rattus norvegicus
-
kidney less activity Rattus norvegicus
-
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
spleen less activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.98
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-15°C, presence of 0.01 mM 2-mercaptoethanol, several weeks, stable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ir
10-formyltetrahydrofolate + NADP+ + H2O calculated equilibrium constant: 160000000, specific for (-)-10-formyltetrahydrofolate and NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ir
additional information
-
 Rattus norvegicus ?
-
 ?
additional information not as substrate: (+)-10-formyltetrahydrofolate Sus scrofa ?
-
 ?
additional information not as substrate: 5-formyltetrahydrofolate Sus scrofa ?
-
 ?
additional information enzyme catalyzes hydrolytic cleavage of 10-formyltetrahydrofolate to formate and tetrahydrofolate in absence of NADP+ (15-30% of the rate of oxidative reaction) Sus scrofa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus
30
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Rattus norvegicus
7.8
-
 broad pH-optimum centers at pH 7.8 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 more than 60% of maximal activity at pH 6.5 and 8.5 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
NAD+ less than 5% of the activity with NADP+ Sus scrofa
NADP+ specific for Rattus norvegicus
NADP+ specific for Sus scrofa
tetrahydrofolate
-
 Rattus norvegicus
tetrahydrofolate
-
 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 tetrahydrofolate (-)-isomer Sus scrofa
0.01
-
 tetrahydrofolate (+)-isomer Sus scrofa