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Literature summary for 1.5.1.54 extracted from

  • Guenther, B.; Sheppard, C.; Tran, P.; Rozen, R.; Matthews, R.; Ludwig, M.
    The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia (1999), Nat. Struct. Biol., 6, 359-365 .
No PubMed abstract available
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Crystallization (Commentary)

Crystallization (Comment) Organism
structure provides a model for the catalytic domain shared by all MTHFRs. This domain is a beta8alpha8 barrel that binds FAD in a special fashion. Ala 177, corresponding to Ala 222 in human MTHFR, is near the bottom of the barrel and distant from the FAD. The mutation A177V does not affect Km or kcat but instead increases the propensity for bacterial MTHFR to lose its essential flavin cofactor Escherichia coli

Protein Variants

Protein Variants Comment Organism
A177V mutation mimicks human polymorphism A222V. Mutation does not affect kcat or the Km values but alters FAD binding Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 5,10-methylenetetrahydrofolate mutant A177V, pH 7.2, 15°C Escherichia coli
0.0039
-
 5,10-methylenetetrahydrofolate wild-type, pH 7.2, 15°C Escherichia coli
0.017
-
 NADH mutant A177V, pH 7.2, 15°C Escherichia coli
0.017
-
 NADH wild-type, pH 7.2, 15°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
 gel filtration, mutant A177V Escherichia coli
119000
-
 gel filtration, wild-type Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEZ1 cf. EC 1.5.1.20
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
 Escherichia coli 5-methyltetrahydrofolate + NAD+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53.8
-
 mutant A177V, melting temperature Escherichia coli
60.9
-
 wild-type, melting temperature Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.5
-
 NADH wild-type, pH 7.2, 15°C Escherichia coli
26.67
-
 NADH mutant A177V, pH 7.2, 15°C Escherichia coli