Crystallization (Comment) | Organism |
---|---|
modeling of the methyltetrahydrofolate product into the enzyme active site and shows 150fold decreased activity | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D120N | mutant is reduced by NADH 30% more rapidly than the wild-type enzyme | Escherichia coli |
E28Q | mutant is unable to catalyze the reduction of 5,10-methylentetrahydrofolate and is inactive in the physiological oxidoreductase reaction. The mutant is able to bind methyltetrahydrofolate, but reduction of the FAD cofactor is not observed | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
5,10-methylenetetrahydrofolate | wild-type, cosubstrate NADH, pH 7.2, 25°C | Escherichia coli | |
0.005 | - |
NADH | mutant D120N, pH 7.2, 25°C | Escherichia coli | |
0.017 | - |
NADH | mutant D120N, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
0.02 | - |
NADH | wild-type, pH 7.2, 25°C | Escherichia coli | |
0.027 | - |
5,10-methylenetetrahydrofolate | mutant D120N, cosubstrate NADH, pH 7.2, 25°C | Escherichia coli | |
0.066 | - |
NADH | wild-type, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
0.08 | - |
NADH | mutant E28Q, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
0.085 | - |
5-methyltetrahydrofolate | wild-type, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
0.106 | - |
5-methyltetrahydrofolate | mutant D120N, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEZ1 | cf. EC 1.5.1.20 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Escherichia coli | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5-methyltetrahydrofolate + menadione | - |
Escherichia coli | 5,10-methylenetetrahydrofolate + menadiol | - |
? | |
NADH + H+ + menadione | - |
Escherichia coli | NAD+ + menadiol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
NADH | mutant E28Q, pH 7.2, 25°C | Escherichia coli | |
0.0002 | - |
menadione | mutant E28Q, pH 7.2, 25°C | Escherichia coli | |
0.0104 | - |
NADH | wild-type, pH 7.2, 25°C | Escherichia coli | |
0.057 | - |
NADH | mutant D120N, pH 7.2, 25°C | Escherichia coli | |
0.087 | - |
menadione | mutant D120N, pH 7.2, 25°C | Escherichia coli | |
0.2 | - |
NADH | mutant E28Q, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
3.2 | - |
menadione | wild-type, pH 7.2, 25°C | Escherichia coli | |
54 | - |
NADH | mutant D120N, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
55 | - |
NADH | wild-type, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADH | wild-type, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli | |
0.04 | - |
NADH | mutant D120N, cosubstrate menadione, pH 7.2, 25°C | Escherichia coli |