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Literature summary for 1.5.1.53 extracted from

  • Matthews, R.G.; Kaufman, S.
    Characterization of the dihydropterin reductase activity of pig liver methylenetetrahydrofolate reductase (1980), J. Biol. Chem., 255, 6014-6017 .
    View publication on PubMed
Search for more literature for 1.5.1.53

Inhibitors

Inhibitors Comment Organism Structure
dihydropteroylhexaglutamate inhibits reduction of quinonoid dihydropterins Sus scrofa
S-adenosyl-L-methionine inhibits reduction of quinonoid dihydropterins Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADPH cosubstrate 5,10-methylenetetrahydrofolate, pH 7.2, 25°C Sus scrofa
0.02
-
 5,10-methylenetetrahydrofolate cosubstrate NADH, pH 7.2, 25°C Sus scrofa
0.02
-
 5,10-methylenetetrahydrofolate cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
0.03
-
 dihydrobiopterin cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
0.04
-
 dihydrofolate cosubstrate NADPH, pH 7.2, 25°C Sus scrofa
0.1
-
 NADH cosubstrate 5,10-methylenetetrahydrofolate, pH 7.2, 25°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
 Sus scrofa 5-methyltetrahydrofolate + NAD+
-
 ?
5,10-methylenetetrahydrofolate + NADPH + H+
-
 Sus scrofa 5-methyltetrahydrofolate + NADP+
-
 ?
6,7-dimethyl-7,8-dihydropterin + NADH + H+
-
 Sus scrofa 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
 ?
dihydrobiopterin + NADPH + H+
-
 Sus scrofa tetrahydrobiopterin + NADP+
-
 ?
dihydrofolate + NADPH + H+
-
 Sus scrofa tetrahydrofolate + NADP+
-
 ?
additional information under Vmax conditions, the turnover numbers for the NADPH-linked reductions of the quinonoid forms of 6,7-dimethyldihydropterin, dihydrobiopterin, and dihydrofolate are all about the same as that for the reduction of methylenetetrahydrofolate. The reduction of quinonoid dihydropterins is inhibited by S-adenosyl-L-methionine and dihydropteroylhexaglutamate Sus scrofa ?
-
-

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is 6fold preferred over NADH Sus scrofa
NADPH
-
 Sus scrofa