Application | Comment | Organism |
---|---|---|
analysis | sensitive radioenzymatic assay that measures directly the reductive catalysis of N ,N -methylenetetrahydrofolate. The radio-labeled substrate, [ C14]N ,N -methyl enetetrahydrofolate, is prepared by condensation of [C14]formaldehyde with tetrahydrofolate. Its stability is maintained for several months by storage at -80°C in a pH 9.5 buffer | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
5,10-methylenetetrahydrofolate | pH 7.5, 23°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | D4A7E8 | cf. EC 1.5.1.20 | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH + H+ | - |
Rattus norvegicus | 5-methyltetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTHFR | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |