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Literature summary for 1.5.1.53 extracted from
- Purification and characterization of methylenetetrahydrofolate reductase from human cadaver liver (1990), Biochem. Med. Metab. Biol., 43, 234-242 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | patients with severe methylenetetrahydrofolate reductase deficiency show neurologic disorders, mental retardation, and rapid deterioration | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-methyltetrahydrofolate | - |
Homo sapiens |  |
| dihydrofolate | - |
Homo sapiens | |
| additional information | not inhibitory: folic acid, S-adenosyl-L-methionine | Homo sapiens | |
| tetrahydrofolate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
NADPH | pH 6.7, 25°C | Homo sapiens | |
| 0.038 | - |
5-methyltetrahydrofolate | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADPH + H+ | Homo sapiens | - |
5-methyltetrahydrofolate + NADP+ | - |
? | |
| 5-methyltetrahydrofolate + oxidized menadione | Homo sapiens | - |
5,10-methylenetetrahydrofolate + reduced menadione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.367 | - |
substrate 5-methyltetrahydrofolate, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADPH + H+ | - |
Homo sapiens | 5-methyltetrahydrofolate + NADP+ | - |
? | |
| 5-methyltetrahydrofolate + oxidized menadione | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? | |
| NADPH + H+ + oxidized menadione | - |
Homo sapiens | NADP+ + reduced menadione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
substrate 5,10-methylenetetrahydrofolate | Homo sapiens |
| 6.6 | - |
substrate 5-methyltetrahydrofolate | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 32% residual activity in absence of FAD | Homo sapiens | |
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Release 2023.1 (January 2023)
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