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Literature summary for 1.5.1.53 extracted from
- Purification and properties of methylenetetrahydrofolate reductase from pig liver (1982), J. Biol. Chem., 257, 140-145.
General Stability
| General Stability | Organism |
|---|---|
| inclusion of 10% glycerol during purification is essential for stability | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
NADPH | - |
Sus scrofa |  |
| 0.019 | - |
5,10-methylenetetrahydrofolate | - |
Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74500 | - |
calculated from amino acid sequence | Sus scrofa |
| 77300 | - |
SDS-PAGE | Sus scrofa |
| 210000 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.4 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, for at least 2 weeks in 10% glycerol | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADPH | - |
Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 77300, SDS-PAGE, gel filtration | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.7 | - |
NADPH | - |
Sus scrofa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Sus scrofa | |
| FAD | each subunit of the dimer or trimer contains bound FAD | Sus scrofa | |
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