Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.5 extracted from

  • Sah, S.; Varshney, U.
    Impact of mutating the key residues of a bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Escherichia coli on its activities (2015), Biochemistry, 54, 3504-3513 .
    View publication on PubMed
Search for more literature for 1.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C58Y kcat/Km for 5,10-methenyltetrahydrofolate is 8.4fold lower than wild-type value Escherichia coli
D121A kcat/Km for 5,10-methenyltetrahydrofolate is 600fold lower than wild-type value Escherichia coli
G122D kcat/Km for 5,10-methenyltetrahydrofolate is 84fold lower than wild-type value Escherichia coli
K54S kcat/Km for 5,10-methenyltetrahydrofolate is identical to wild-type value Escherichia coli
Q98K very low activity with 5,10-methenyltetrahydrofolate Escherichia coli
R191E very low activity with 5,10-methenyltetrahydrofolate Escherichia coli
Y50S very low activity with 5,10-methenyltetrahydrofolate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
0.104
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
0.187
-
 NADP+ pH 7.6, 30°C, wild-type enzyme Escherichia coli
0.205
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
0.233
-
 NADP+ pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
0.279
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli
0.302
-
 NADP+ pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
0.302
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P24186
-
-
Escherichia coli K12 P24186
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Escherichia coli 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+
-
 Escherichia coli K12 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
0.06
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
2.6
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
2.8
-
 NADP+ pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
12.3
-
 NADP+ pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
14.3
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
17.08
-
 NADP+ pH 7.6, 30°C, wild-type enzyme Escherichia coli
19.9
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.096
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A Escherichia coli
0.88
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D Escherichia coli
8.6
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
9.2
-
 NADP+ pH 7.6, 30°C, mutant enzyme C58Y Escherichia coli
52.7
-
 NADP+ pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
69.8
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme K54S Escherichia coli
71.3
-
 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme Escherichia coli
91.3
-
 NADP+ pH 7.6, 30°C, wild-type enzyme Escherichia coli