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Literature summary for 1.5.1.5 extracted from

  • Eadsforth, T.C.; Cameron, S.; Hunter, W.N.
    The crystal structure of Leishmania major N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase and assessment of a potential drug target (2012), Mol. Biochem. Parasitol., 181, 178-185.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania major

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M ammonium acetate Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
LY354899 i.e. 5,6,7,8-tetrahydro N5,N10-carbonylfolic acid Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31900
-
 2 * 31900, calculated from amino acid sequence Leishmania major
65000
-
 gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+ Leishmania major
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r
5,10-methylenetetrahydrofolate + NADP+ Leishmania major Fridlin
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Leishmania major Q4Q9F9
-
-
Leishmania major Fridlin Q4Q9F9
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Leishmania major 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r
5,10-methylenetetrahydrofolate + NADP+
-
 Leishmania major Fridlin 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 31900, calculated from amino acid sequence Leishmania major

Synonyms

Synonyms Comment Organism
DHCH
-
 Leishmania major
N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase bifunctional enzyme Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Leishmania major

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000105
-
 LY354899 pH and temperature not specified in the publication Leishmania major