Literature summary for 1.5.1.5 extracted from

Pawelek, P.D.; MacKenzie, R.E.
Methenyltetrahydrofolate cyclohydrolase is rate limiting for the enzymatic conversion of 10-formyltetrahydrofolate to 5,10-methylenetetrahydrofolate in bifunctional dehydrogenase-cyclohydrolase enzymes (1998), Biochemistry, 37, 1109-1115.

Search for more literature for 1.5.1.5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	5,10-methylenetetrahydrofolate	deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
0.009	-	5,10-methylenetetrahydrofolate	NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
0.01	-	5,10-methylenetetrahydrofolate	bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme	Photobacterium phosphoreum
0.012	-	5,10-methylenetetrahydrofolate	NAD+-M2+-phopsphate-dependent mitochondrial dehydrogenase-cyclohydrolase enzyme	Homo sapiens
0.022	-	NADP+	NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
0.038	-	NADP+	bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme	Photobacterium phosphoreum
0.507	-	NAD+	NAD+-M2+-phopsphate-dependent mitochondrial dehydrogenase-cyclohydrolase enzyme	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + NADP+	Photobacterium phosphoreum	-	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Photobacterium phosphoreum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + NADP+	-	Photobacterium phosphoreum	5,10-methenyltetrahydrofolate + NADPH + H+	-	?
5,10-methylenetetrahydrofolate + NADP+	deuterated folate substrate also	Homo sapiens	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	5,10-methylenetetrahydrofolate	deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
3.8	-	NADP+	substrate constant: methylenetetrahydrofolate, deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
9.4	-	5,10-methylenetetrahydrofolate	NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens
10	-	NADP+	NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Homo sapiens
NADP+	-	Photobacterium phosphoreum

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Release 2023.1 (January 2023)