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Literature summary for 1.5.1.5 extracted from
- Enzymes of clostridial purine fermentation. Methylenetetrahydrofolate dehydrogenase (1967), J. Biol. Chem., 242, 4378-4385.
General Stability
| General Stability | Organism |
|---|---|
| upon daily freezing and thawing activity loss of 30% in 2 weeks | Clostridium cylindrosporum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Clostridium cylindrosporum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0088 | - |
5,10-methylenetetrahydropteroyltriglutamate | - |
Clostridium cylindrosporum | |
| 0.026 | - |
5,10-methylenetetrahydrofolate | - |
Clostridium cylindrosporum | |
| 0.034 | - |
NADP+ | - |
Clostridium cylindrosporum | |
| 1.6 | - |
5,10-Methylenetetrahydropteroate | - |
Clostridium cylindrosporum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
sucrose density gradient centrifugation | Clostridium cylindrosporum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | Clostridium cylindrosporum | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium cylindrosporum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium cylindrosporum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Clostridium cylindrosporum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable fot at least 2 months | Clostridium cylindrosporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Clostridium cylindrosporum | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
| 5,10-methylenetetrahydropteroate + NADP+ | - |
Clostridium cylindrosporum | 5,10-methenyltetrahydropteroate + NADPH | - |
? | |
| 5,10-methylenetetrahydropteroyltriglutamate + NADP+ | - |
Clostridium cylindrosporum | 5,10-methenyltetrahydropteroyltriglutamate + NADPH | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | half-maximal activities at pH 5 and 9.8 | Clostridium cylindrosporum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Clostridium cylindrosporum | |
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Release 2023.1 (January 2023)
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