Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.5 extracted from

  • Pawelek, P.D.; MacKenzie, R.E.
    Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue (1996), Biochim. Biophys. Acta, 1296, 47-54.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate effect on the NAD+-dependent dehydrogenase activity Photobacterium phosphoreum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Photobacterium phosphoreum

Inhibitors

Inhibitors Comment Organism Structure
potassium phosphate effect on NADP+-dependent dehydrogenase activity Photobacterium phosphoreum
potassium sulfate effect on NADP+-dependent dehydrogenase activity Photobacterium phosphoreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
5,10-methylenetetrahydrofolate cofactor: NADP+ Photobacterium phosphoreum
0.016
-
5,10-methylenetetrahydrofolate cofactor: NAD+ Photobacterium phosphoreum
0.2
-
NADP+
-
Photobacterium phosphoreum
4.76
-
5,10-methylenetetrahydrofolate
-
Photobacterium phosphoreum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Photobacterium phosphoreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+ Photobacterium phosphoreum
-
5,10-methenyltetrahydrofolate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Photobacterium phosphoreum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase Photobacterium phosphoreum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97.4
-
recombinant bifunctional enzyme Photobacterium phosphoreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NAD+
-
Photobacterium phosphoreum 5,10-methenyltetrahydrofolate + NADH + H+
-
r
5,10-methylenetetrahydrofolate + NADP+
-
Photobacterium phosphoreum 5,10-methenyltetrahydrofolate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Photobacterium phosphoreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
113
-
NADP+
-
Photobacterium phosphoreum
136
-
NAD+
-
Photobacterium phosphoreum

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ preferred cofactor in vivo Photobacterium phosphoreum
NADP+
-
Photobacterium phosphoreum