Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | effect on the NAD+-dependent dehydrogenase activity | Photobacterium phosphoreum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Photobacterium phosphoreum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
potassium phosphate | effect on NADP+-dependent dehydrogenase activity | Photobacterium phosphoreum | |
potassium sulfate | effect on NADP+-dependent dehydrogenase activity | Photobacterium phosphoreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
5,10-methylenetetrahydrofolate | cofactor: NADP+ | Photobacterium phosphoreum | |
0.016 | - |
5,10-methylenetetrahydrofolate | cofactor: NAD+ | Photobacterium phosphoreum | |
0.2 | - |
NADP+ | - |
Photobacterium phosphoreum | |
4.76 | - |
5,10-methylenetetrahydrofolate | - |
Photobacterium phosphoreum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Photobacterium phosphoreum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | Photobacterium phosphoreum | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photobacterium phosphoreum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase | Photobacterium phosphoreum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97.4 | - |
recombinant bifunctional enzyme | Photobacterium phosphoreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NAD+ | - |
Photobacterium phosphoreum | 5,10-methenyltetrahydrofolate + NADH + H+ | - |
r | |
5,10-methylenetetrahydrofolate + NADP+ | - |
Photobacterium phosphoreum | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Photobacterium phosphoreum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
113 | - |
NADP+ | - |
Photobacterium phosphoreum | |
136 | - |
NAD+ | - |
Photobacterium phosphoreum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ preferred cofactor in vivo | Photobacterium phosphoreum | |
NADP+ | - |
Photobacterium phosphoreum |