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Literature summary for 1.5.1.46 extracted from
- New insights into ergot alkaloid biosynthesis in Claviceps purpurea: an agroclavine synthase EasG catalyses, via a non-enzymatic adduct with reduced glutathione, the conversion of chanoclavine-I aldehyde to agroclavine (2011), Org. Biomol. Chem., 9, 4328-4335.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| reduced glutathione | required for activity | Claviceps purpurea |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BXL1 Blue MRF cells | Claviceps purpurea |
| expressed in Escherichia coli strain XL1 Blue MRF | Claviceps purpurea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
| 30000 | - |
x * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
| 31900 | - |
1 * 31900, native enzyme, calculated from amino acid sequence | Claviceps purpurea |
| 31900 | - |
x * 31900, calculated from amino acid sequence | Claviceps purpurea |
| 33300 | - |
1 * 33300, His-tagged enzyme, calculated from amino acid sequence | Claviceps purpurea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Claviceps purpurea | - |
- |
- |
| Claviceps purpurea | P0CT21 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Claviceps purpurea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chanoclavine-I aldehyde + NADH + H+ | 54% conversion compared to NADPH, in the presence of reduced glutathione | Claviceps purpurea | agroclavine + NAD+ | - |
? | |
| chanoclavine-I aldehyde + NADH + H+ | the reaction occurs only in the presence of reduced glutathione (conversion rate of 54%) | Claviceps purpurea | agroclavine + NAD+ | - |
? | |
| chanoclavine-I aldehyde + NADPH + H+ | the reaction occurs only in the presence of reduced glutathione or beta-mercaptoethanol or dithiothreitol | Claviceps purpurea | agroclavine + NADP+ | - |
? | |
| chanoclavine-I aldehyde + NADPH + H+ | the reaction occurs only in the presence of the thiolation agents reduced glutathione (conversion rate of 59%) or 2-mercaptoethanol (conversion rate of 99%) and dithiothreitol (conversion rate of 67%). No product formation is detected in the incubation mixture with L-ascorbic acid instead of GSH | Claviceps purpurea | agroclavine + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
| ? | x * 31900, calculated from amino acid sequence | Claviceps purpurea |
| monomer | 1 * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
| monomer | 1 * 31900, native enzyme, calculated from amino acid sequence | Claviceps purpurea |
| monomer | 1 * 33300, His-tagged enzyme, calculated from amino acid sequence | Claviceps purpurea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agroclavine synthase | - |
Claviceps purpurea |
| EasG | - |
Claviceps purpurea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Claviceps purpurea | |
| NADPH | - |
Claviceps purpurea | |
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