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Literature summary for 1.5.1.45 extracted from

  • Liu, X.; Dong, Y.; Li, X.; Ren, Y.; Li, Y.; Wang, W.; Wang, L.; Feng, L.
    Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2 (2010), Microbiology, 156, 589-595.
    View publication on PubMed
Search for more literature for 1.5.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
-
-
Geobacillus thermodenitrificans NG80-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chelating Sepharose column chromatography Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55.47
-
 using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C Geobacillus thermodenitrificans
58.2
-
 using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+ 95% activity with NADH relative to NADPH Geobacillus thermodenitrificans FADH2 + NAD+
-
 ?
FAD + NADPH + H+ higher activity is obtained with FAD than with FMN using NADPH as the cofactor Geobacillus thermodenitrificans FADH2 + NADP+
-
 ?
FMN + NADPH + H+ higher activity is obtained with FAD than with FMN using NADPH as the cofactor Geobacillus thermodenitrificans FMNH2 + NADP+
-
 ?
additional information anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP Geobacillus thermodenitrificans ?
-
 ?

Synonyms

Synonyms Comment Organism
FAD reductase
-
 Geobacillus thermodenitrificans
GTNG_3158
-
 Geobacillus thermodenitrificans