Literature summary for 1.5.1.44 extracted from

Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.
Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3 (2010), Org. Biomol. Chem., 8, 3500-3508.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL1 Blue MRF cells	Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	1 * 30000, His6-tagged enzyme, SDS-PAGe	Aspergillus fumigatus
33300	-	1 * 33300, calculated from amino acid sequence	Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	-	-
Aspergillus fumigatus Af293	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chanoclavine-I aldehyde + NADH + H+	the reaction occurs in the presence of the old yellow enzyme FgaOx3	Aspergillus fumigatus	festuclavine + NAD+	-	?
chanoclavine-I aldehyde + NADH + H+	the reaction occurs in the presence of the old yellow enzyme FgaOx3	Aspergillus fumigatus Af293	festuclavine + NAD+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 30000, His6-tagged enzyme, SDS-PAGe	Aspergillus fumigatus
monomer	1 * 33300, calculated from amino acid sequence	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
festuclavine synthase	-	Aspergillus fumigatus
FgaFS	-	Aspergillus fumigatus

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Release 2023.1 (January 2023)