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Literature summary for 1.5.1.41 extracted from

  • Okai, M.; Ohtsuka, J.; Asano, A.; Guo, L.; Miyakawa, T.; Miyazono, K.; Nakamura, A.; Okada, A.; Zheng, H.; Kimura, K.; Nagata, K.; Tanokura, M.
    High pressure refolding, purification, and crystallization of flavin reductase from Sulfolobus tokodaii strain 7 (2012), Protein Expr. Purif., 84, 214-218.
    View publication on PubMed
Search for more literature for 1.5.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expressed of N-terminally His-tagged HpaCSt protein in Escherichia coli Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the refolded enzyme are obtained by adding FMN, FAD, or riboflavin to the protein solution and without the addition of flavin compound Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-
Sulfurisphaera tokodaii 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH + H+
-
 Sulfurisphaera tokodaii FMNH2 + NAD+
-
 ?
FMN + NADH + H+
-
 Sulfurisphaera tokodaii 7 FMNH2 + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
flavin reductase HpaCSt
-
 Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
FMN the purified refolded enzyme does not contain FMN, unlike the same enzyme expressed as a soluble protein. After the addition of FMN to the protein solution, the refolded enzyme shows a higher activity than the enzyme expressed as the soluble protein Sulfurisphaera tokodaii