Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.40 extracted from

  • Kunow,J.; Schwörer, B.; Stetter, K.O.; Thauer, R.K.
    A F420-dependent NADP reductase in the extremely thermophilie sulfate-reducing Archaeoglobus fulgidus (1993), Arch. Microbiol., 160, 199-205.
No PubMed abstract available
Search for more literature for 1.5.1.40

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited by iodoacetamide (20 mM), chloromercuribenzoate (20 mM) or N-ethylmaleimide (7 mM) when preincubated for 30 min in the presence of these reagents. The enzyme is not inactivated by dioxygen Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 oxidized coenzyme F420 pH 5.5, 65°C Archaeoglobus fulgidus
0.02
-
 reduced coenzyme F420 pH 8.0, 65°C Archaeoglobus fulgidus
0.04
-
 NADP+ pH 8.0, 65°C Archaeoglobus fulgidus
0.04
-
 NADPH pH 5.5, 65°C Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Archaeoglobus fulgidus
52000
-
 sedimentation analysis Archaeoglobus fulgidus
57000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced coenzyme F420 + NADP+ Archaeoglobus fulgidus
-
 oxidized coenzyme F420 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29370
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
660
-
 pH 8.0, 80°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced coenzyme F420 + NADP+
-
 Archaeoglobus fulgidus oxidized coenzyme F420 + NADPH + H+
-
 r
reduced coenzyme F420 + NADP+ the enzyme is Si face specific with respect to C5 of reduced coenzyme F420 and Si face specific with respect to C4 of NADP+. The enzyme is specific for both coenzyme F420 and NADP+/NADPH Archaeoglobus fulgidus oxidized coenzyme F420 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
F420-dependent NADP reductase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 reduction of oxidized coenzyme F420 with NADPH Archaeoglobus fulgidus
8
-
 reduction of NADP+ with reduced coenzyme F420 Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 45% of maximal activity, pH 6.5: about 35% of maximal activity, reduction of oxidized coenzyme F420 with NADPH Archaeoglobus fulgidus
7 9 pH 7.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity, reduction of NADP+ with reduced coenzyme F420 Archaeoglobus fulgidus