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Literature summary for 1.5.1.39 extracted from
- Structural and functional study of ChuY from Escherichia coli strain CFT073 (2017), Biochem. Biophys. Res. Commun., 482, 1176-1182 .
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | enzyme ChuY is a potential target for the development of antibacterial or antivirulence drugs to combat not only UPEC but a broad range of pathogenic bacteria that contain chuY system or its orthologue, ranging from Vibrio to staphylococcal species | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chuY, encoded in the chu gene cluster of strain CFT073, recombinant expression as His-tagged enzyme in Escherichia coli, complementation of an Escherichia coli chuY-knockout strain with expression of the wild-type gene | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged enzyme, using 0.1 M Bis-Tris, pH 6.5, 50 mM CaCl2, and 30% PEG MME 550 as precipitants, ChuY crystals belong to the primitive monoclinic space group P21 with two molecules forming an asymmetric unit, multi-wavelength anomalous dispersion, using a SeMet-labeled crystal at a resolution of 2.4 A | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chuY-knockout strain | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMNH2 + NAD(P)+ | Escherichia coli | - |
FMN + NAD(P)H + H+ | - |
r |  |
| FMNH2 + NAD(P)+ | Escherichia coli CFT073 | - |
FMN + NAD(P)H + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A0A0H2VDD2 | a non-infectious strain | - |
| Escherichia coli CFT073 | A0A0H2VDD2 | a non-infectious strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMNH2 + NAD(P)+ | - |
Escherichia coli | FMN + NAD(P)H + H+ | - |
r | |
| FMNH2 + NAD(P)+ | - |
Escherichia coli CFT073 | FMN + NAD(P)H + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | ChuY exists as a monomer in solution, SDS-PAGE and gel filtration | Escherichia coli |
| More | the two molecules in the asymmetric unit are related by pseudo 2fold rotation symmetry. ChuY contains six alpha-helices and ten beta-strands. A central beta-sheet, consisting of seven parallel beta-strands, beta1, beta2, beta3, beta4, beta5, beta6, and beta10, is flanked by six alpha-helices, forming alternating beta-strand and alpha-helix repeats, which is a representative feature of Rossmann folds. Three other beta-strands (beta7-beta9) are located on the top of the beta-sheet | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChuY | - |
Escherichia coli |
| flavin mononucleotide reductase | - |
Escherichia coli |
| FMN reductase | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | the enzyme has a hemin binding activity, pronbably in the hydrophobic pocket near loops beta4-alpha5 and beta8-beta9, docking of biliverdin IXalpha and FMN , overview | Escherichia coli | |
| additional information | enzyme ChuY binds nucleotides as cofactors., cofactor binding structure, overview | Escherichia coli | |
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADP+ | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | gene chuY is highly conserved in a broad range of pathogenic bacteria | Escherichia coli |
| malfunction | a chuY deletion-insertion strain shows reduced survival potential compared to wild-type and complemented strains in mammalian cells | Escherichia coli |
| physiological function | ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of Escherichia coli strain CFT073 | Escherichia coli |
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