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Literature summary for 1.5.1.38 extracted from

  • Jablonski, E.; DeLuca, M.
    Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi (1977), Biochemistry, 16, 2932-2936.
    View publication on PubMed
Search for more literature for 1.5.1.38

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.005 M, 14% inhibition Vibrio harveyi
AMP 0.01 M, 28% inhibition Vibrio harveyi
dicoumarol 0.000325 M, 15% inhibition Vibrio harveyi
KCN 0.01 M, 10% inhibition Vibrio harveyi
N-ethylmaleimide 0.001 M, 26% inhibition Vibrio harveyi
p-hydroxymercuribenzoate 0.001 M, 80% inhibition Vibrio harveyi
rotenone 0.0004 M, 30% inhibition Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 FMN pH 7.0, 23°C, spectrometric assay Vibrio harveyi
0.018
-
 FMN pH 5.6, 23°C, spectrometric assay Vibrio harveyi
0.02
-
 NADPH pH 5.6, 23°C, spectrometric assay Vibrio harveyi
0.04
-
 NADPH pH 7.0, 23°C Vibrio harveyi
0.04
-
 NADPH pH 7.0, 23°C, spectrometric assay Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?
FMN + NADPH + H+ Vibrio harveyi No. 392
-
 FMNH2 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-
Vibrio harveyi No. 392
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio harveyi

Reaction

Reaction Comment Organism Reaction ID
FMNH2 + NADP+ = FMN + NADPH + H+ the enzyme exhibits double displacement or ping-pong kinetics Vibrio harveyi
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
 pH 7.0, 23°C Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADPH + H+
-
 Vibrio harveyi FADH2 + NADP+
-
 ?
FAD + NADPH + H+
-
 Vibrio harveyi No. 392 FADH2 + NADP+
-
 ?
FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
FMN + NADPH + H+ the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi FMNH2 + NADP+
-
 ?
FMN + NADPH + H+
-
 Vibrio harveyi No. 392 FMNH2 + NADP+
-
 ?
FMN + NADPH + H+ the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi No. 392 FMNH2 + NADP+
-
 ?
additional information the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor Vibrio harveyi ?
-
 ?
additional information the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor Vibrio harveyi No. 392 ?
-
 ?

Synonyms

Synonyms Comment Organism
NADPH specific FMN reductase
-
 Vibrio harveyi
NADPH:FMN oxidoreductase
-
 Vibrio harveyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34
-
 NADPH pH 7.0, 23°C Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Vibrio harveyi
7
-
 assay at Vibrio harveyi

pH Range

pH Minimum pH Maximum Comment Organism
5 10 the enzyme exhibits a maximum activity at pH 5.5 which drops to a broad shoulder from pH 6.5 to pH 8.5 with an activity 75% that of maximum at pH 7.0. About 60% of maximal activity at pH 5.0, about 50% of maximal activity at pH 10.0 Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
NADPH the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi