Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19400 | - |
deduced from cDNA, without His-tag but with FAD | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the reductase is purified as a stable C-terminally His-tagged yellow protein containing weakly bound FAD | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | the reductase catalyzes the reduction of FAD by NADH and releases the FADH- product into solution, but unlike the reductase from Actinetobacter baumannii, this catalysis is not influenced by p-hydroxyphenylacetate | Pseudomonas aeruginosa | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | HpaC can reduce either FAD or FMN | Pseudomonas aeruginosa | FMNH2 + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
flavin reductase | - |
Pseudomonas aeruginosa |
HpaC | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme solated with some FAD | Pseudomonas aeruginosa | |
NADH | no activity with NADPH | Pseudomonas aeruginosa |