Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptomyces globisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
FAD | pH 7.5, 22°C | Streptomyces globisporus | |
0.053 | - |
NADH | pH 7.5, 22°C | Streptomyces globisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | Streptomyces globisporus | the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 | FADH2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces globisporus | Q8GME2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces globisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 | Streptomyces globisporus | FADH2 + NAD+ | - |
? | |
FAD + NADH + H+ | strictly NADH- and FAD-specific enzyme: No activity with NADPH or FMN | Streptomyces globisporus | FADH2 + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SgcE6 | - |
Streptomyces globisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Streptomyces globisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
NADH | pH 7.5, 22°C | Streptomyces globisporus | |
4.5 | - |
FAD | pH 7.5, 22°C | Streptomyces globisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces globisporus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 | Streptomyces globisporus |