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Literature summary for 1.5.1.37 extracted from

  • Van Lanen, S.G.; Lin, S.; Horsman, G.P.; Shen, B.
    Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027 (2009), FEMS Microbiol. Lett., 300, 237-241.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces globisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
FAD pH 7.5, 22°C Streptomyces globisporus
0.053
-
NADH pH 7.5, 22°C Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FAD + NADH + H+ Streptomyces globisporus the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 FADH2 + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus Q8GME2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+ the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 Streptomyces globisporus FADH2 + NAD+
-
?
FAD + NADH + H+ strictly NADH- and FAD-specific enzyme: No activity with NADPH or FMN Streptomyces globisporus FADH2 + NAD+
-
?

Synonyms

Synonyms Comment Organism
SgcE6
-
Streptomyces globisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Streptomyces globisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
NADH pH 7.5, 22°C Streptomyces globisporus
4.5
-
FAD pH 7.5, 22°C Streptomyces globisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces globisporus

General Information

General Information Comment Organism
physiological function the enzyme can adequately supply reduced flavin under saturating substrate conditions to support catalysis of flavin-dependent halogenase SgcC3 and monooxygenase SgcC during biosynthesis of C-1027 Streptomyces globisporus