Literature summary for 1.5.1.36 extracted from

Yeo, Y.J.; Shin, S.; Lee, S.G.; Park, S.; Jeong, Y.J.
Production, purification, and characterization of soluble NADH-flavin Oxidoreductase (StyB) from Pseudomonas putida SN1 (2009), J. Microbiol. Biotechnol., 19, 362-367.

Search for more literature for 1.5.1.36

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli strain BL21(DE3)pLysS effectively produces an active and soluble form of StyB as about 9% of the total protein content, when cultivated at 20°C with 0.5 mM IPTG	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	NADH	pH 7.2, 37°C	Pseudomonas putida
0.056	-	FAD	pH 7.2, 37°C	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18000	-	SDS-PAGE	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
FAD + NADH + H+	-	Pseudomonas putida	FADH2 + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
NADH-flavin oxidoreductase	-	Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at, StyB activity increases at temperatures up to 37°C, and decreases thereafter	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Pseudomonas putida

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	7.5	optimal range	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas putida
NADH	no activity with NADPH	Pseudomonas putida

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Release 2023.1 (January 2023)