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Literature summary for 1.5.1.34 extracted from

  • Kiefer, P.M.; Grimshaw, C.E.; Whiteley, J.M.
    The comparative interaction of quinonoid (6R)-Dihydrobiopterin and an alternative dihydropterin substrate with wild-type and mutant rat dihydropteridine reductases (1997), Biochemistry, 36, 9438-9445.
    View publication on PubMed
Search for more literature for 1.5.1.34

Protein Variants

Protein Variants Comment Organism
A133S
-
 Rattus norvegicus
Y146F
-
 Rattus norvegicus
Y146F/A133S almost no activity with quinonoid 6,7-dimethyldihydropteridine, higher activity with quinoid dihydrobipterin than wild-type Rattus norvegicus
Y146F/K150E no activity with quinonoid 6,7-dimethyldihydropteridine, higher activity with quinoid dihydrobipterin than wild-type Rattus norvegicus
Y146H
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 quinonoid dihydrobiopterin recombinant wild-type enzyme Rattus norvegicus
0.0004
-
 quinonoid dihydrobiopterin A133S mutant enzyme Rattus norvegicus
0.002
-
 NADH Y146F/A133S mutant enzyme Rattus norvegicus
0.003
-
 NADH Y146F mutant enzyme Rattus norvegicus
0.007
-
 quinonoid dihydrobiopterin Y146H mutant enzyme Rattus norvegicus
0.008
-
 NADH Y146H mutant enzyme Rattus norvegicus
0.008
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine A133S mutant enzyme Rattus norvegicus
0.013
-
 NADH recombinant wild-type enzyme Rattus norvegicus
0.013
-
 NADH K150E mutant enzyme Rattus norvegicus
0.022
-
 NADH A133S mutant enzyme Rattus norvegicus
0.024
-
 quinonoid dihydrobiopterin Y146F/A133S mutant enzyme Rattus norvegicus
0.027
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine recombinant wild-type enzyme Rattus norvegicus
0.028
-
 quinonoid dihydrobiobiopterin K150E mutant enzyme Rattus norvegicus
0.1
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146F/A133S mutant enzyme Rattus norvegicus
0.17
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146H mutant enzyme Rattus norvegicus
0.29
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine K150E mutant enzyme Rattus norvegicus
0.308
-
 quinonoid dihydrobiopterin Y146F mutant enzyme Rattus norvegicus
0.46
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146F mutant enzyme Rattus norvegicus
1
-
 quinonoid dihydrobiopterin Y146F/K150E mutant enzyme, more than Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinoid dihydrobiopterin + NAD(P)H
-
 Rattus norvegicus tetrahydrobiopterin + NAD(P)+
-
 ?
quinonoid 6,7-dimethyl-7,8-dihydropteridine + NADH + H+
-
 Rattus norvegicus 6,7-dimethyl-5,6,7,8-tetrahydropteridine + NAD+
-
 r
quinonoid dihydrobiopterin + NADH + H+
-
 Rattus norvegicus 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146F/A133S mutant enzyme Rattus norvegicus
17
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146F mutant enzyme Rattus norvegicus
20
-
 quinoid dihydrobiopterin A133S mutant enzyme Rattus norvegicus
23
-
 quinoid dihydrobiopterin recombinant wild-type enzyme Rattus norvegicus
36
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine Y146H mutant enzyme Rattus norvegicus
52
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine K150E mutant enzyme Rattus norvegicus
74
-
 quinoid dihydrobiopterin Y146F/A133S mutant enzyme Rattus norvegicus
138
-
 quinoid dihydrobiopterin Y146F mutant enzyme Rattus norvegicus
139
-
 quinoid dihydrobiopterin Y146H mutant enzyme Rattus norvegicus
152
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine A133S mutant enzyme Rattus norvegicus
153
-
 quinonoid 6,7-dimethyl-7,8-dihydropteridine recombinant wild-type enzyme Rattus norvegicus
200
-
 quinoid dihydrobiopterin Y146F/K150E mutant enzyme, approx. value Rattus norvegicus
269
-
 quinoid dihydrobiobiopterin K150E mutant enzyme Rattus norvegicus