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Literature summary for 1.5.1.33 extracted from

  • Ong, H.B.; Sienkiewicz, N.; Wyllie, S.; Fairlamb, A.H.
    Dissecting the metabolic roles of pteridine reductase 1 in Trypanosoma brucei and Leishmania major (2011), J. Biol. Chem., 286, 10429-10438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.33

Inhibitors

Inhibitors Comment Organism Structure
dihydrobiopterin inhibits the enzyme at high substrate concentrations Leishmania major
dihydrobiopterin inhibits the enzyme at high substrate concentrations Trypanosoma brucei
quinonoid 6,7-dimethyl-7,8-dihydropterin
-
 Leishmania major
quinonoid 6,7-dimethyl-7,8-dihydropterin
-
 Trypanosoma brucei
quinonoid dihydrobiopterin
-
 Leishmania major
quinonoid dihydrobiopterin
-
 Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000294
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.0032
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.0036
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.0103
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.0166
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.021
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trypanosoma brucei 5829
-
cytosol
-
 Leishmania major 5829
-

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biopterin + NADPH + H+
-
 Trypanosoma brucei dihydrobiopterin + NADP+
-
 ?
biopterin + NADPH + H+
-
 Leishmania major dihydrobiopterin + NADP+
-
 ?
dihydrobiopterin + NADPH + H+
-
 Trypanosoma brucei 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
dihydrobiopterin + NADPH + H+
-
 Leishmania major 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 Trypanosoma brucei ?
-
 ?
quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 Leishmania major ?
-
 ?
quinonoid dihydrobiopterin + NADPH + H+
-
 Trypanosoma brucei tetrahydrobiopterin + NADP+
-
 ?
quinonoid dihydrobiopterin + NADPH + H+
-
 Leishmania major tetrahydrobiopterin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
pteridine reductase 1
-
 Trypanosoma brucei
pteridine reductase 1
-
 Leishmania major
PTR1
-
 Trypanosoma brucei
PTR1
-
 Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.43
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.44
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.54
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.57
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.84
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8
-
 Trypanosoma brucei
5.5 8
-
 Leishmania major

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
 Trypanosoma brucei
4 9
-
 Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Trypanosoma brucei
NADPH
-
 Leishmania major

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.00116
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.00122
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.00314
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
0.0922
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
0.475
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei

General Information

General Information Comment Organism
physiological function the enzyme has an essential and dual role in pterin metabolism Trypanosoma brucei
physiological function the enzyme has an essential and dual role in pterin metabolism Leishmania major

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
21
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
26
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
160
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
170
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
180
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1700
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei