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Literature summary for 1.5.1.33 extracted from
- Trypanosoma brucei pteridine reductase 1 is essential for survival in vitro and for virulence in mice (2010), Mol. Microbiol., 77, 658-671.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pteridine reductase 1 | - |
Trypanosoma brucei |
| PTR1 | - |
Trypanosoma brucei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | impossible to generate PTR1 null mutants. RNA interference results in complete knockdown of endogenous protein after 48 h, followed by cell death after 4 days. Lethal phenotype is reversed by expression of PTR1 in RNAi lines or by addition of tetrahydrobiopterin to cultures. Loss of PTR1 is associated with gross morphological changes due to a defect in cytokinesis, resulting in cells with multiple nuclei and kinetoplasts, as well as multiple detached flagella. Electron microscopy also reveal increased numbers of glycosomes, while immunofluorescence microscopy show increased and more diffuse staining for glycosomal matrix enzymes, indicative of mis-localisation to the cytosol. RNAi cell lines are markedly less virulent than wild-type parasites in mice | Trypanosoma brucei |
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