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Literature summary for 1.5.1.33 extracted from

  • Dawson, A.; Gibellini, F.; Sienkiewicz, N.; Tulloch, L.B.; Fyfe, P.K.; McLuskey, K.; Fairlamb, A.H.; Hunter, W.N.
    Structure and reactivity of Trypanosoma brucei pteridine reductase: inhibition by the archetypal antifolate methotrexate (2006), Mol. Microbiol., 61, 1457-1468.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
dihydrobiopterin substrate inhibition Trypanosoma brucei
methotrexate
-
Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
dihydrobiopterin pH 3.7 Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
pH 3.7, substrate dihydrofolate Trypanosoma brucei
0.1
-
pH 3.7, substrate folate Trypanosoma brucei
2
-
pH 3.7, substrate biopterin Trypanosoma brucei
2.3
-
pH 3.7, substrate dihydrobiopterin Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH
-
Trypanosoma brucei 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
dihydrobiopterin + NADPH
-
Trypanosoma brucei 5,6,7,8-tetrahydrobiopterin + NADP+
-
?
Dihydrofolate + NADPH
-
Trypanosoma brucei 5,6,7,8-Tetrahydrofolate + NADP+
-
?
folate + NADPH
-
Trypanosoma brucei 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer crystallization data Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
dihydrobiopterin pH 3.7 Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7
-
-
Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000152
-
methotrexate pH 3.7 Trypanosoma brucei
0.0038
-
dihydrobiopterin pH 3.7 Trypanosoma brucei