Literature summary for 1.5.1.33 extracted from

Zhao, H.; Bray, T.; Ouellette, M.; Zhao, M.; Ferre, R.A.; Matthews, D.; Whiteley, J.M.; Varughese, K.I.
Structure of pteridine reductase (PTR1) from Leishmania tarentolae (2003), Acta Crystallogr. Sect. D, 59, 1539-1544.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Leishmania tarentolae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in binary complex with NADPH and in ternary complex with NADPH and methotrexate, 0.02 ml of protein solution containing 20 mg/ml protein, 20 mM Tris-HCl, pH 7.0, mixed with 0.01 ml of a solution containing 4 mM NADPH, 4 mM methotrexate on ice for 1 h, then mixed with 0.01 ml of reservoir solution containing 28% 1,4-butanediol, 12.5 mM cetyl trimethyl ammonium chloride and 100 mM HEPES, pH 7.0, 5 days, 14°C, X-ray diffraction structure determination and analysis of flash frozen crystals at 2.8 A resolution, molecular replacement technique	Leishmania tarentolae

Crystallization (Comment)

Organism

purified recombinant enzyme in binary complex with NADPH and in ternary complex with NADPH and methotrexate, 0.02 ml of protein solution containing 20 mg/ml protein, 20 mM Tris-HCl, pH 7.0, mixed with 0.01 ml of a solution containing 4 mM NADPH, 4 mM methotrexate on ice for 1 h, then mixed with 0.01 ml of reservoir solution containing 28% 1,4-butanediol, 12.5 mM cetyl trimethyl ammonium chloride and 100 mM HEPES, pH 7.0, 5 days, 14°C, X-ray diffraction structure determination and analysis of flash frozen crystals at 2.8 A resolution, molecular replacement technique

Leishmania tarentolae

Inhibitors

Inhibitors	Comment	Organism	Structure
methotrexate	binding structure	Leishmania tarentolae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Leishmania tarentolae	enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania tarentolae	P42556	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Leishmania tarentolae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Biopterin + NADPH	2-step reaction via intermediate 7,8-dihydrobiopterin	Leishmania tarentolae	5,6,7,8-Tetrahydrobiopterin + NADP+	-	?
additional information	enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway	Leishmania tarentolae	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	structure analysis	Leishmania tarentolae

Synonyms

Synonyms	Comment	Organism
pteridine reductase	-	Leishmania tarentolae
PTR1	-	Leishmania tarentolae

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	binding structure	Leishmania tarentolae