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Literature summary for 1.5.1.31 extracted from

  • Galanie, S.; Smolke, C.
    Optimization of yeast-based production of medicinal protoberberine alkaloids (2015), Microb. Cell Fact., 14, 144 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis heterologous production of berberine and the optimization of the engineered biosynthetic pathway from rac-norlaudanosoline to (S)-canadine in yeast involving the recombinant berberine reductase Berberis wilsoniae

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized gene yBwSTOX is expressed in Saccharomyces cerevisiae from plasmid pCS3203, coexpression with 6 other enzyme required for production of protoberberine Berberis wilsoniae

Protein Variants

Protein Variants Comment Organism
additional information recombinant coexpression of enzyme STOX and six other enzymes, including the three membrane-bound enzymes: the flavin-dependent oxidase berberine bridge enzyme, the cytochrome P450 canadine synthase, and a cytochrome P450 reductase, in Saccharomyces cerevisiae results in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor rac-norlaudanosoline. Improvement of flux through the pathway, by methods including enzyme variant screening, genetic copy number variation, and culture optimization, leading to an over 70fold increase in canadine titer up to 1.8 mg/l Berberis wilsoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-canadine + 2 NADP+ Berberis wilsoniae
-
berberine + 2 NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Berberis wilsoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-canadine + 2 NADP+
-
Berberis wilsoniae berberine + 2 NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
(S)-tetrahydroprotoberberine oxidase
-
Berberis wilsoniae
STOX
-
Berberis wilsoniae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Berberis wilsoniae