Application | Comment | Organism |
---|---|---|
synthesis | heterologous production of berberine and the optimization of the engineered biosynthetic pathway from rac-norlaudanosoline to (S)-canadine in yeast involving the recombinant berberine reductase | Berberis wilsoniae |
Cloned (Comment) | Organism |
---|---|
codon-optimized gene yBwSTOX is expressed in Saccharomyces cerevisiae from plasmid pCS3203, coexpression with 6 other enzyme required for production of protoberberine | Berberis wilsoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant coexpression of enzyme STOX and six other enzymes, including the three membrane-bound enzymes: the flavin-dependent oxidase berberine bridge enzyme, the cytochrome P450 canadine synthase, and a cytochrome P450 reductase, in Saccharomyces cerevisiae results in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor rac-norlaudanosoline. Improvement of flux through the pathway, by methods including enzyme variant screening, genetic copy number variation, and culture optimization, leading to an over 70fold increase in canadine titer up to 1.8 mg/l | Berberis wilsoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-canadine + 2 NADP+ | Berberis wilsoniae | - |
berberine + 2 NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Berberis wilsoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-canadine + 2 NADP+ | - |
Berberis wilsoniae | berberine + 2 NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(S)-tetrahydroprotoberberine oxidase | - |
Berberis wilsoniae |
STOX | - |
Berberis wilsoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Berberis wilsoniae |