Cloned (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
General Stability | Organism |
---|---|
stable at temperatures below 50°C | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-hydroxycoumarin | - |
Rhodococcus erythropolis | |
Ag+ | - |
Rhodococcus erythropolis | |
Cu2+ | - |
Rhodococcus erythropolis | |
Hg+ | - |
Rhodococcus erythropolis | |
NEM | when the enzyme is preincubated with NEM and NADH in the absence of FMN | Rhodococcus erythropolis | |
p-chloromercuribenzoic acid | - |
Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0108 | - |
FMN | - |
Rhodococcus erythropolis | |
0.208 | - |
NADH | - |
Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
4 * 22000, SDS-PAGE | Rhodococcus erythropolis |
86000 | - |
gel filtration | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
D1 | - |
Rhodococcus erythropolis D1 | - |
D1 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
122 | - |
- |
Rhodococcus erythropolis |
133 | - |
flavin reductase from recombinant Escherichia coli | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Rhodococcus erythropolis | FMNH2 + NADP+ | - |
? | |
FMN + NADPH | - |
Rhodococcus erythropolis D1 | FMNH2 + NADP+ | - |
? | |
riboflavin-5-phosphate + NADH + H+ | - |
Rhodococcus erythropolis | reduced FMN + NAD+ | - |
r | |
riboflavin-5-phosphate + NADH + H+ | - |
Rhodococcus erythropolis D1 | reduced FMN + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 22000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
enzyme assay | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme assay | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | without any chromophore like flavin, NADPH is inert | Rhodococcus erythropolis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00338 | - |
7-hydroxycoumarin | - |
Rhodococcus erythropolis |