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Literature summary for 1.5.1.30 extracted from
- Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase (1998), Biosci. Biotechnol. Biochem., 62, 1978-1987.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00085 | - |
NADPH | nitrofurazone as electron acceptor | Bacillus subtilis |  |
| 0.0035 | - |
NADPH | FMN as electron accceptor | Bacillus subtilis | |
| 0.0047 | - |
FMN | - |
Bacillus subtilis | |
| 0.0163 | - |
nitrofurazone | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Bacillus subtilis |
| 28320 | - |
calculated from sequence of cDNA | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
ISW 1214. | - |
| Bacillus subtilis ISW 1214. | - |
ISW 1214. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 53 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | - |
Bacillus subtilis | FMNH2 + NADP+ | - |
? | |
| FMN + NADPH | - |
Bacillus subtilis ISW 1214. | FMNH2 + NADP+ | - |
? | |
| nitrofurazone + NADPH + 4 H+ | - |
Bacillus subtilis | 5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O | - |
? | |
| nitrofurazone + NADPH + 4 H+ | - |
Bacillus subtilis ISW 1214. | 5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
enzyme assay | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Bacillus subtilis | |
| NADPH | - |
Bacillus subtilis | |
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