Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
FMN | - |
Vibrio harveyi | |
0.02 | - |
NADPH | - |
Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26320 | - |
calculated from sequence of cloned DNA | Vibrio harveyi |
28000 | - |
1 * 28000, wild type and cloned, SDS-PAGE | Vibrio harveyi |
33000 | - |
gel filtration | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Vibrio harveyi MAV / ATCC 33843 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FMN-agarose chromatography as a key step | Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
88 | - |
- |
Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
FMN + NADPH | - |
Vibrio harveyi MAV / ATCC 33843 | FMNH2 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, wild type and cloned, SDS-PAGE | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
NADPH:flavin oxidoreductase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
enzyme assay | Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36 | - |
FMN | - |
Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | tightly bound | Vibrio harveyi |