General Stability | Organism |
---|---|
freezing and thawing causes no appreciable loss of the enzyme activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
2,3-diphosphoglycerate | - |
Homo sapiens | |
acrinol | - |
Homo sapiens | |
Bathocuproine sulfonate | - |
Homo sapiens | |
Cu2+ | - |
Homo sapiens | |
HgCl2 | - |
Homo sapiens | |
NADP+ | 0.1 mM, about 50% inhibition at no inhibition by NAD+ at pH 7.5, 12% inhibition at pH 4.8 | Homo sapiens | |
PCMB | - |
Homo sapiens | |
proflavin hemisulfate | - |
Homo sapiens | |
SeO32- | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Fe2+, Fe3+, Mg2+, and Ca2+ do not affect the enzyme activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21500 | - |
x * 21500, SDS-PAGE | Homo sapiens |
22000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.37 | - |
pH 7.0, 25°C | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, the purified enzyme is stable at | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH + H+ | - |
Homo sapiens | FMNH2 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21500, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NADPH-FMN reductase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
boiling of the enzyme leads to complete loss of the FMN reductase activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 85% of maximal activity, pH 8.0: about 45% of maximal activity | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the FMN reductase activity is highly specific for NADPH, and the activity with NADH is 10% of that with NADPH | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | - |
- |
8.1 |