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Literature summary for 1.5.1.30 extracted from

  • Yubisui, T.; Matsuki, T.; Takeshita, M.; Yoneyama, Y.
    Characterization of the purified NADPH-flavin reductase of human erythrocytes (1979), J. Biochem., 85, 719-728.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing causes no appreciable loss of the enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
2,3-diphosphoglycerate
-
Homo sapiens
acrinol
-
Homo sapiens
Bathocuproine sulfonate
-
Homo sapiens
Cu2+
-
Homo sapiens
HgCl2
-
Homo sapiens
NADP+ 0.1 mM, about 50% inhibition at no inhibition by NAD+ at pH 7.5, 12% inhibition at pH 4.8 Homo sapiens
PCMB
-
Homo sapiens
proflavin hemisulfate
-
Homo sapiens
SeO32-
-
Homo sapiens
Zn2+
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Fe2+, Fe3+, Mg2+, and Ca2+ do not affect the enzyme activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
x * 21500, SDS-PAGE Homo sapiens
22000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.37
-
pH 7.0, 25°C Homo sapiens

Storage Stability

Storage Stability Organism
4°C, the purified enzyme is stable at Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH + H+
-
Homo sapiens FMNH2 + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 21500, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NADPH-FMN reductase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
boiling of the enzyme leads to complete loss of the FMN reductase activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH 4.5: about 85% of maximal activity, pH 8.0: about 45% of maximal activity Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH the FMN reductase activity is highly specific for NADPH, and the activity with NADH is 10% of that with NADPH Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
-
8.1