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Literature summary for 1.5.1.3 extracted from
- Structure and kinetics assays of recombinant Schistosoma mansoni dihydrofolate reductase (2017), Acta Trop., 170, 190-196 .
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is an anti-parasitic drug target, e.g. for malaria or human cancers, because rapidly growing cells require folate to produce thymine | Schistosoma mansoni |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Smdhfr, single-copy gene, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Schistosoma mansoni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged enzyme, mixing of 0.001 ml of 8 mg/ml protein solution with 0.001 ml of well solution containing 100 mM Tris-HCl, pH 8.0, and 2.0 M ammonium sulfate, at 18°C, two weeks, X-ray diffraction structure determination and analysis at 1.95 A resolution, molecular replacement using the modified structure of chicken liver DHFR, PDB ID 8DFR, as search model | Schistosoma mansoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | a folate analogue that is recognized as folate competitor, used as chemotherapeutic agent in cancer and autoimmune diseases | Schistosoma mansoni |  |
| methotrexate | a folate analogue that is recognized as folate competitor, used as chemotherapeutic agent in cancer and autoimmune diseases | Schistosoma mansoni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0264 | - |
7,8-dihydrofolate | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
| 0.123 | - |
NADP+ | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH + H+ | Schistosoma mansoni | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | G4VJD6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Schistosoma mansoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH + H+ | - |
Schistosoma mansoni | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| additional information | substrate binding site analysis, overview | Schistosoma mansoni | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22000, recombinant enzyme, SDS-PAGE, 1 * 21089, sequence calculation | Schistosoma mansoni |
| More | the DHFR enzyme structure exhibits the canonical DHFR fold | Schistosoma mansoni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHFR | - |
Schistosoma mansoni |
| Smdhfr | - |
Schistosoma mansoni |
| Smp 175230 | - |
Schistosoma mansoni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Schistosoma mansoni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35.9 | - |
NADP+ | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
| 130 | - |
7,8-dihydrofolate | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Schistosoma mansoni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | binding site analysis, overview | Schistosoma mansoni | |
| NADPH | - |
Schistosoma mansoni | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | alignment analysis shows several substitutions between Schistosoma mansoni and human DHFR at both the folate- and NADP+-binding sites. The folate-binding site exhibits two differences at three residues (human DHFR numbering): E30D and N64F. The NADP+-binding site exhibits six substitutions at 11 residues: D21G,K54R, K55V, R76S, E77T and S118Y | Schistosoma mansoni |
| metabolism | dihydrofolate reductase (DHFR) is involved in the thymidylate and is essential for nucleotide metabolism | Schistosoma mansoni |
| additional information | the DHFR enzyme structure exhibits the canonical DHFR fold | Schistosoma mansoni |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 210.56 | - |
NADP+ | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
| 4924.24 | - |
7,8-dihydrofolate | pH 7.8, 25°C, recombinant enzyme | Schistosoma mansoni | |
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