Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HB101 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
DELTAAla145 | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAArg52 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAArg52/DELTAAla145 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAArg52/DELTAGly121 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAArg52/DELTAGly67 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAGly121 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAGly121/DELTAAla145 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAGly67 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAGly67/DELTAAla145 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
DELTAGly67/DELTAGly121 | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
7,8-dihydrofolate | deletion mutant DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
0.0013 | - |
7,8-dihydrofolate | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
0.0048 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
0.0051 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
0.0056 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
0.0073 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
0.0108 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
0.0502 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
0.0512 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
0.0777 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
0.1161 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52, at pH 7.0 and 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Escherichia coli | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABQ4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
0.37 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
0.38 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
1.7 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
16.5 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
23.1 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
24 | - |
7,8-dihydrofolate | deletion mutant DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
24.8 | - |
7,8-dihydrofolate | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
31.9 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
41.1 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
96.3 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52, at pH 7.0 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
30 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
70 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
160 | - |
7,8-dihydrofolate | deletion mutant DELTAGly121, at pH 7.0 and 25°C | Escherichia coli | |
410 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
460 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
830 | - |
7,8-dihydrofolate | deletion mutant DELTAArg52, at pH 7.0 and 25°C | Escherichia coli | |
3300 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67, at pH 7.0 and 25°C | Escherichia coli | |
8600 | - |
7,8-dihydrofolate | deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C | Escherichia coli | |
19200 | - |
7,8-dihydrofolate | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
29400 | - |
7,8-dihydrofolate | deletion mutant DELTAAla145, at pH 7.0 and 25°C | Escherichia coli |