Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) pLysS | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
modified microbatch method with PRG 4000 as the primary precipitating agent, crystals belong to the orthorhombic space group P2(1)2(1)2(1), with two molecules per asymmetric unit and 52% solvent content | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Plasmodium falciparum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | a major band at 35000 Da and minor bands at 35000 Da and 42000 Da detected on SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
dihydrofolate reductase | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Plasmodium falciparum |