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Literature summary for 1.5.1.3 extracted from
- Purification and characterization of recombinant Thermotoga maritima dihydrofolate reductase (1998), Eur. J. Biochem., 255, 628-637.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | recombinant enzyme, 1.4fold activation at 40-200 mM | Thermotoga maritima |  |
| NaCl | recombinant enzyme, 1.4fold activation at 40-200 mM | Thermotoga maritima | |
| Urea | recombinant enzyme, 7 M | Thermotoga maritima | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
General Stability
| General Stability | Organism |
|---|---|
| substrates 7,8-dihydrofolate and NADPH stabilize against thermal inactivation | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | recombinant enzyme, above 20 mM | Thermotoga maritima | |
| Guanidine-HCl | recombinant enzyme, 0.5 M | Thermotoga maritima | |
| methotrexate | recombinant enzyme | Thermotoga maritima | |
| Sodium dodecyl sulfate | recombinant enzyme, 0.1 M | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
7,8-dihydrofolate | recombinant enzyme | Thermotoga maritima | |
| 0.004 | - |
NADPH | recombinant enzyme | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy | Thermotoga maritima |
| 36000 | - |
about, recombinant enzyme, gel filtration | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
thermophilic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 173 | - |
recombinant enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Thermotoga maritima | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: about 60 min, half-life with NADPH: more than 360 min, half-life with 7,8-dihydrofolate: more than 240 min, half-life with 1M urea: 26-28 min | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme-dihydrofolate complex is soluble at basic pH and precipitates reversibly at acidic pH | Thermotoga maritima |
| 6.3 | - |
recombinant enzyme, 60°C | Thermotoga maritima |
| 6.5 | - |
recombinant enzyme, 37°C | Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Thermotoga maritima | |
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