Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Heliothis virescens | |
p-chloromercuribenzoate | - |
Heliothis virescens |
Application | Comment | Organism |
---|---|---|
medicine | potentially target for the discovery of novel insecticides and anthelminthics | Heliothis virescens |
Cloned (Comment) | Organism |
---|---|
amplification and DNA-sequencing of full length gene from DNA-library | Heliothis virescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | complete inhibition at 0.0001 mM, slow tight binding inhibitor | Heliothis virescens | |
p-hydroxymercuribenzoate | - |
Heliothis virescens | |
pyrimethamine | anti-malarial DHFR inhibitor | Heliothis virescens | |
Sodium tetrathionate | thiol-blocking reagent | Heliothis virescens | |
trimethoprim | - |
Heliothis virescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Drosophila melanogaster | - |
- |
soluble | at low levels in the cytosol | Heliothis virescens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | 21000 | DNA-sequence dtermination and SDS-PAGE | Heliothis virescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Heliothis virescens | - |
i.e. Helicoverpa virescens, tobacco budworm | - |
Purification (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
- |
Heliothis virescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | substrate and antifolate inhibitor methotrexate binding, modeling of three-dimensional structure | Heliothis virescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Heliothis virescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.8 | - |
purified enzyme | Heliothis virescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | strongly specific for | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | strongly specific for | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | no activity | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | no activity | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific | Drosophila melanogaster | |
NADPH | specific | Heliothis virescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000003 | - |
methotrexate | - |
Heliothis virescens | |
0.000021 | - |
pyrimethamine | - |
Heliothis virescens | |
0.00024 | - |
trimethoprim | - |
Heliothis virescens |