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Literature summary for 1.5.1.3 extracted from
- Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver (1966), J. Biol. Chem., 241, 1319-1328.
General Stability
| General Stability | Organism |
|---|---|
| N5-formyltetrahydrofolate, NADP+ and NADPH stabilize and protect against heat inactivation | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | - |
Gallus gallus |  |
| citrate | in acid pH range: inhibitor of reduction of 7,8-dihydrofolate but not folate | Gallus gallus | |
| NADP+ | - |
Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00015 | - |
7,8-dihydrofolate | with NADPH | Gallus gallus | |
| 0.0067 | - |
folate | - |
Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
sedimentation equilibrium studies, sucrose density gradient experiments, aminopterin titration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, 0.02 mM NADPH, 3 months, frequent freezing and thawing, stable | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 2 min | Gallus gallus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
7,8-dihydrofolate | pH 7.4 | Gallus gallus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
folate + NADPH | Gallus gallus |
| 4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4 and pH 7.4 | Gallus gallus |
| 5.2 | 5.5 | 7,8-dihydrofolate + NADH | Gallus gallus |
| 7.4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4.0 and pH 7.4 | Gallus gallus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | maximal stability | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | chicken liver: at neutral pH specific for NADPH and 7,8-dihydrofolate, at acid pH: NADH + 7,8-dihydrofolate and folate + NADPH | Gallus gallus | |
| NADPH | - |
Gallus gallus | |
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