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Literature summary for 1.5.1.3 extracted from
- Purification and characterization of dihydrofolate reductase from Walker 256 carcinosarcoma (1982), Can. J. Biochem., 60, 1132-1142.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 3 to 5fold increase in activity | Rattus norvegicus |  |
| additional information | no activation by NaCl, Na2SO4, MgCl2, Li2SO4, CaCl2, MgSO4 | Rattus norvegicus | |
| Urea | slight activation | Rattus norvegicus | |
General Stability
| General Stability | Organism |
|---|---|
| 4°C, without urea, 50% loss of activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | - |
Rattus norvegicus | |
| chaotrophes | e.g. guanidine-HCl | Rattus norvegicus | |
| Guanidine-HCl | above 0.25 M | Rattus norvegicus | |
| methotrexate | - |
Rattus norvegicus | |
| N10-formylaminopterin | - |
Rattus norvegicus | |
| Triazinate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
7,8-dihydrofolate | - |
Rattus norvegicus | |
| 0.029 | - |
NADPH | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21500 | - |
sedimentation equilibrium ultracentrifugation | Rattus norvegicus |
| 22500 | - |
Walker 256 carcinoma cells, meniscus depletion method | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
carcinoma Walker 256 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Walker 256 carcinosarcoma cells | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| LLC-WRC 256 cell | tumor cell line | Rattus norvegicus | - |
| Walker carcinoma 256 cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
pH 7.3 | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -65°C, frozen tissue, 4 months | Rattus norvegicus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 2 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
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