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Literature summary for 1.5.1.3 extracted from

  • Gupta, R.S.; Flintoff, W.F.; Siminovitch, L.
    Purification and properties of dihydrofolate reductase from methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells (1977), Can. J. Biochem., 55, 445-452.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Cricetulus griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
ovary cells, SDS-PAGE Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
chinese hamster, methotrexate-sensitive and methotrexate-resistant cells
-

Purification (Commentary)

Purification (Comment) Organism
methotrexate-sensitive and methotrexate resistant cells Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.75
-
methotrexate-sensitive strain Cricetulus griseus
0.85
-
methotrexate-resistant strain Cricetulus griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
wild-type: decay rate constant 0.12 per min, mutant enzymes 0.06-0.08 per min Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
Cricetulus griseus