Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Daucus carota | |
aminopterin | - |
Daucus carota | |
methotrexate | - |
Daucus carota | |
N-ethylmaleimide | - |
Daucus carota | |
N10-formylaminopterin | - |
Daucus carota | |
p-hydroxymercuribenzoate | - |
Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, dihydrofolate reductase subunit, SDS-PAGE | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
wild carrot, enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function | - |
Purification (Comment) | Organism |
---|---|
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function | Daucus carota |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
purified enzyme | Daucus carota |
Subunits | Comment | Organism |
---|---|---|
More | enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, 286000 MW complex, 45000 MW dihydrofolate reductase subunit and 4 other polypeptide chains: 95000, 70000, 50000, 26000 | Daucus carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
assay at | Daucus carota |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
assay at pH 7.2, no activity at pH 5.9 | Daucus carota |