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Literature summary for 1.5.1.3 extracted from
- Deoxyribonucleotide biosynthesis in green algae: characterization of thymidylate synthase-dihydrofolate reductase in Scenedesmus obliquus (1987), Arch. Biochem. Biophys., 256, 244-252.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| folate | - |
Tetradesmus obliquus |  |
| methotrexate | - |
Tetradesmus obliquus | |
| additional information | thymidylate synthase activity inhibitors | Tetradesmus obliquus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE | Tetradesmus obliquus |
| 100000 | - |
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation | Tetradesmus obliquus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetradesmus obliquus | - |
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | Tetradesmus obliquus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.8 | - |
- |
Tetradesmus obliquus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, enzyme after affinity chromatography, half-life 2 days | Tetradesmus obliquus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Tetradesmus obliquus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE | Tetradesmus obliquus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Tetradesmus obliquus |
| 25 | - |
assay at | Tetradesmus obliquus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | Tetradesmus obliquus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity negligible below and above | Tetradesmus obliquus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Tetradesmus obliquus | |
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Release 2023.1 (January 2023)
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