Application | Comment | Organism |
---|---|---|
medicine | enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer | Mycolicibacterium phlei |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes purified enzyme | Mycolicibacterium phlei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Mycolicibacterium phlei | |
trimethoprim | - |
Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
7,8-dihydrofolate | - |
Mycolicibacterium phlei | |
0.035 | - |
NADPH | - |
Mycolicibacterium phlei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
SDS-PAGE | Mycolicibacterium phlei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Mycolicibacterium phlei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycolicibacterium phlei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
3.5 h, no loss of activity in presence of bovine serum albumin | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycolicibacterium phlei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycolicibacterium phlei |