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Literature summary for 1.5.1.3 extracted from

  • Al-Rubeai, M.; Dale, J.W.
    Purification and characterization of dihydrofolate reductase from Mycobacterium phlei (1986), Biochem. J., 235, 301-303.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Mycolicibacterium phlei

General Stability

General Stability Organism
bovine serum albumin stabilizes purified enzyme Mycolicibacterium phlei

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Mycolicibacterium phlei
trimethoprim
-
Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
7,8-dihydrofolate
-
Mycolicibacterium phlei
0.035
-
NADPH
-
Mycolicibacterium phlei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
SDS-PAGE Mycolicibacterium phlei

Organism

Organism UniProt Comment Textmining
Mycolicibacterium phlei
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Mycolicibacterium phlei 5,6,7,8-tetrahydrofolate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mycolicibacterium phlei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
3.5 h, no loss of activity in presence of bovine serum albumin Mycolicibacterium phlei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mycolicibacterium phlei

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mycolicibacterium phlei