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Literature summary for 1.5.1.3 extracted from
- Dihydrofolate reductase from soybean seedlings. Characterization of the enzyme purified by affinity chromatography (1976), Arch. Biochem. Biophys., 174, 675-683.
General Stability
| General Stability | Organism |
|---|---|
| 7,8-dihydrofolate protects against heat inactivation | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Glycine max |  |
| aminopterin | - |
Glycine max | |
| methotrexate | - |
Glycine max | |
| N-ethylmaleimide | - |
Glycine max | |
| N10-formylaminopterin | - |
Glycine max | |
| p-hydroxymercuribenzoate | - |
Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
7,8-dihydrofolate | - |
Glycine max | |
| 0.415 | - |
NADPH | - |
Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| enzyme denatured with 8 M urea cannot be renatured by removal of urea | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156.3 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Glycine max | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | polymer, SDS-PAGE | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
7,8-dihydrofolate protects against heat inactivation | Glycine max |
| 55 | - |
5 min, 70% loss of activity, 15 min, more than 80% loss of activity | Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
7,8-dihydrofolate | - |
Glycine max | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Glycine max | |
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Release 2023.1 (January 2023)
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