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Literature summary for 1.5.1.3 extracted from

  • Joyner, S.S.; Fling, M.E.; Stone, D.; Baccanari, D.P.
    Characterization of an R-plasmid dihydrofolate reductase with a monomeric structure (1984), J. Biol. Chem., 259, 5851-5856.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Salmonella enterica subsp. enterica serovar Typhimurium
trimethoprim
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.0004
-
7,8-dihydrofolate
-
Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
overview Salmonella enterica subsp. enterica serovar Typhimurium
16700
-
PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium
16900
-
1 * 16900, R-plasmid enzyme, PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
phage type 179, R-plasmid enzyme, isozyme type III
-

Purification (Commentary)

Purification (Comment) Organism
phage type 179, R-plasmid enzyme, isozyme type III Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, pH 7, 20% glycerol, 1 mM EDTA, 1 mM dithiothreitol, at least 90% of activity retained, 2-3 weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Salmonella enterica subsp. enterica serovar Typhimurium 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 16900, R-plasmid enzyme, PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
7.8
-
R-plasmid encoded enzyme, 2 optima: pH 4.2 and pH 7.8 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information overview Salmonella enterica subsp. enterica serovar Typhimurium
0.000000009
-
methotrexate isozyme type III, R-plasmid encoded Salmonella enterica subsp. enterica serovar Typhimurium
0.000019
-
trimethoprim isozyme type III, R-plasmid encoded Salmonella enterica subsp. enterica serovar Typhimurium