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Literature summary for 1.5.1.3 extracted from

  • Ferone, R.; Roland, S.
    Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata (1980), Proc. Natl. Acad. Sci. USA, 77, 5802-5806.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
bovine serum albumin, 1 mg/ml, stabilizes during storage of purified enzyme preparation at 5°C Crithidia fasciculata
bovine serum albumin, 1 mg/ml, stabilizes during storage of purified enzyme preparation at 5°C Plasmodium berghei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56700
-
2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE Crithidia fasciculata
107000
-
gel filtration Crithidia fasciculata
107000
-
bifunctional enzyme: thymidylate synthase-dihydrofolate reductase Crithidia fasciculata

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45
-
Plasmodium berghei
-
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45
-

Purification (Commentary)

Purification (Comment) Organism
dihydrofolate reductase-thymidylate synthase EC 2.1.1.45 Crithidia fasciculata
methotrexate-affinity chromatography Crithidia fasciculata
methotrexate-affinity chromatography Plasmodium berghei

Storage Stability

Storage Stability Organism
5°C, 50 mM Tris-HCl, pH 8.3, 1 mM EDTA, 1 mM dithiothreitol, 1 M KCl, 0.6 mM 7,8-dihydrofolate, 50% loss of activity overnight Plasmodium berghei

Subunits

Subunits Comment Organism
dimer 2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE Crithidia fasciculata

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Crithidia fasciculata