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Literature summary for 1.5.1.3 extracted from
- Presence and properties of dihydrofolate reductases within the genus Crithidia (1969), Biochim. Biophys. Acta, 178, 453-458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| folate | at 0.1 mM | Crithidia fasciculata |  |
| folate | at 0.1 mM | Strigomonas oncopelti | |
| methotrexate | - |
Crithidia fasciculata | |
| methotrexate | - |
Strigomonas oncopelti | |
| additional information | no effect of biopterin and dihydrobiopterin at 0.1 mM; overview | Crithidia fasciculata | |
| additional information | no effect of biopterin and dihydrobiopterin at 0.1 mM; overview | Strigomonas oncopelti | |
| pyrimethamine | - |
Crithidia fasciculata | |
| pyrimethamine | - |
Strigomonas oncopelti | |
| trimethoprim | - |
Crithidia fasciculata | |
| trimethoprim | - |
Strigomonas oncopelti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Crithidia fasciculata | |
| additional information | - |
additional information | - |
Strigomonas oncopelti | |
| 0.003 | - |
7,8-dihydrofolate | - |
Crithidia fasciculata | |
| 0.003 | - |
7,8-dihydrofolate | - |
Strigomonas oncopelti | |
| 0.05 | - |
NADPH | - |
Crithidia fasciculata | |
| 0.05 | - |
NADPH | - |
Strigomonas oncopelti | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
| Strigomonas oncopelti | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH + H+ | - |
Strigomonas oncopelti | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| folate + NADPH | no activity | Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | no activity | Strigomonas oncopelti | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| additional information | no activity with 7,8-dihydrobiopterin and biopterin | Crithidia fasciculata | ? | - |
? | |
| additional information | no activity with 7,8-dihydrobiopterin and biopterin | Strigomonas oncopelti | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Crithidia fasciculata |
| 6 | 7 | - |
Strigomonas oncopelti |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.8 | half-maximal activities at pH 5.5 and pH 8.8 | Crithidia fasciculata |
| 5.5 | 7.8 | half-maximal activities at pH 5.5 and pH 7.8 | Strigomonas oncopelti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | not | Crithidia fasciculata | |
| NADH | not | Strigomonas oncopelti | |
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